| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Leukocyte Biology, Vol 61, Issue 2 147-155, Copyright © 1997 by Society for Leukocyte Biology
JOURNAL ARTICLE |
HA Doyle and JW Murphy
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.
Leukocyte infiltration into infected tissues is essential for the clearance of microorganisms. In animals with a cell-mediated immune (CMI) response to the infectious agent, as opposed to naive animals, leukocyte migration is greatly enhanced into sites of the organism or antigen. The role of the,chemotactic cytokine or chemokine, macrophage inflammatory protein-1 alpha (MIP-1 alpha), in the expression phase of the CMI response and in protection against Cryptococcus neoformans was assessed. With the use of a gelatin sponge model in mice as a means of detecting an anti-cryptococcal delayed-type hypersensitivity (DTH) reaction, we found that MIP-1 alpha levels in fluids from cryptococcal antigen (CneF)-injected sponges in immunized mice (DTH-reactive sponges) were significantly increased over levels of MIP-1 alpha in fluids from saline-injected control sponges at 12 and 24-30 h after injection. MIP-1 alpha levels peaked before increases in neutrophils and lymphocytes in the DTH-reactive sponges, suggesting that MIP-1 alpha was responsible, at least in part, for attracting these leukocyte types. Immunized mice treated with neutralizing antibody to MIP-1 alpha before sponge injection with CneF had reduced numbers of neutrophils and lymphocytes in the DTH-reactive sponges and showed reduced clearance of C. neoformans from the lungs, spleens, livers, and brains when compared with controls. Furthermore, injection of rmMIP-1 alpha into sponges in naive mice resulted in an increase in the influx of neutrophils and lymphocytes into the sponges compared with saline-injected sponges. Together our findings provide solid evidence that MIP-1 alpha is a component of the anticryptococcal DTH reaction. In addition, MIP-1 alpha influences neutrophil influx and attracts lymphocytes into the DTH reaction site. Finally, we showed that MIP-1 alpha plays a role in protection against C. neoformans.
This article has been cited by other articles:
|
|
 |
|
  C. D. L. Ramos, C. Canetti, J. T. Souto, J. S. Silva, C. M. Hogaboam, S. H. Ferreira, and F. Q. Cunha MIP-1{alpha}[CCL3] acting on the CCR1 receptor mediates neutrophil migration in immune inflammation via sequential release of TNF-{alpha} and LTB4 J. Leukoc. Biol., July 1, 2005; 78(1): 167 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Bauman, G. B. Huffnagle, and J. W. Murphy Effects of Tumor Necrosis Factor Alpha on Dendritic Cell Accumulation in Lymph Nodes Draining the Immunization Site and the Impact on the Anticryptococcal Cell-Mediated Immune Response Infect. Immun., January 1, 2003; 71(1): 68 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Tumpey, R. Fenton, S. Molesworth-Kenyon, J. E. Oakes, and R. N. Lausch Role for Macrophage Inflammatory Protein 2 (MIP-2), MIP-1{alpha}, and Interleukin-1{alpha} in the Delayed-Type Hypersensitivity Response to Viral Antigen J. Virol., July 17, 2002; 76(16): 8050 - 8057. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. L. Ma and C. H. Mody When There Is a Fungus Among Us, What Makes it Virulent? Am. J. Respir. Cell Mol. Biol., March 1, 2002; 26(3): 273 - 276. [Full Text] [PDF]
|
|
|
|
|
|
K. L. Nichols, S. K. Bauman, F. B. Schafer, and J. W. Murphy Differences in Components at Delayed-Type Hypersensitivity Reaction Sites in Mice Immunized with Either a Protective or a Nonprotective Immunogen of Cryptococcus neoformans Infect. Immun., February 1, 2002; 70(2): 591 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Lindell, T. J. Standiford, P. Mancuso, Z. J. Leshen, and G. B. Huffnagle Macrophage Inflammatory Protein 1{alpha}/CCL3 Is Required for Clearance of an Acute Klebsiella pneumoniae Pulmonary Infection Infect. Immun., October 1, 2001; 69(10): 6364 - 6369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. D. Hazlett, S. McClellan, R. Barrett, and X. Rudner B7/CD28 Costimulation Is Critical in Susceptibility to Pseudomonas aeruginosa Corneal Infection: A Comparative Study Using Monoclonal Antibody Blockade and CD28-Deficient Mice J. Immunol., January 15, 2001; 166(2): 1292 - 1299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Olszewski, G. B. Huffnagle, R. A. McDonald, D. M. Lindell, B. B. Moore, D. N. Cook, and G. B. Toews The Role of Macrophage Inflammatory Protein-1{alpha}/CCL3 in Regulation of T Cell-Mediated Immunity to Cryptococcus neoformans Infection J. Immunol., December 1, 2000; 165(11): 6429 - 6436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. McGaha and J. W. Murphy CTLA-4 Down-Regulates the Protective Anticryptococcal Cell-Mediated Immune Response Infect. Immun., August 1, 2000; 68(8): 4624 - 4630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mehrad, T. A. Moore, and T. J. Standiford Macrophage Inflammatory Protein-1{alpha} Is a Critical Mediator of Host Defense Against Invasive Pulmonary Aspergillosis in Neutropenic Hosts J. Immunol., July 15, 2000; 165(2): 962 - 968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Bauman, K. L. Nichols, and J. W. Murphy Dendritic Cells in the Induction of Protective and Nonprotective Anticryptococcal Cell-Mediated Immune Responses J. Immunol., July 1, 2000; 165(1): 158 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yoshimoto, C.-R. Wang, T. Yoneto, A. Matsuzawa, W. W. Cruikshank, and H. Nariuchi Role of IL-16 in delayed-type hypersensitivity reaction Blood, May 1, 2000; 95(9): 2869 - 2874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Huffnagle, L. K. McNeil, R. A. McDonald, J. W. Murphy, G. B. Toews, N. Maeda, and W. A. Kuziel Cutting Edge: Role of C-C Chemokine Receptor 5 in Organ-Specific and Innate Immunity to Cryptococcus neoformans J. Immunol., November 1, 1999; 163(9): 4642 - 4646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. S. Aliberti, F. S. Machado, J. T. Souto, A. P. Campanelli, M. M. Teixeira, R. T. Gazzinelli, and J. S. Silva beta -Chemokines Enhance Parasite Uptake and Promote Nitric Oxide-Dependent Microbiostatic Activity in Murine Inflammatory Macrophages Infected with Trypanosoma cruzi Infect. Immun., September 1, 1999; 67(9): 4819 - 4826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Doyle and J. W. Murphy Role of the C-C Chemokine, TCA3, in the Protective Anticryptococcal Cell-Mediated Immune Response J. Immunol., April 15, 1999; 162(8): 4824 - 4833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Murphy, F. Schafer, A. Casadevall, and A. Adesina Antigen-Induced Protective and Nonprotective Cell-Mediated Immune Components against Cryptococcus neoformans Infect. Immun., June 1, 1998; 66(6): 2632 - 2639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Tumpey, H. Cheng, D. N. Cook, O. Smithies, J. E. Oakes, and R. N. Lausch Absence of Macrophage Inflammatory Protein-1alpha Prevents the Development of Blinding Herpes Stromal Keratitis J. Virol., May 1, 1998; 72(5): 3705 - 3710. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
