| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Leukocyte Biology, Vol 61, Issue 2 224-230, Copyright © 1997 by Society for Leukocyte Biology
JOURNAL ARTICLE |
G Cox and RC Austin
Father Sean O'Sullivan Research Centre, St. Joseph's Hospital, Hamilton, Ontario, Canada.
We examined the mechanisms of corticosteroid inhibition of cell death by apoptosis in human neutrophils. Suppression of apoptosis by dexamethasone was abolished by co-treatment with the protein synthesis inhibitor cycloheximide. At doses of 1 microg/mL cycloheximide did not reduce basal survival of neutrophils but effectively inhibited dexamethasone-induced increases in 24-h survival (24.4 +/- 8.7 vs. 49.6 +/- 10%, P < 0.01). Similar results were obtained with actinomycin D, an inhibitor of mRNA synthesis. The factor(s) responsible for mediating increased survival following dexamethasone treatment is not active extracellularly because dexamethasone-treated neutrophil-conditioned medium (CM) had no effect on the survival of naive neutrophils when the direct effects of dexamethasone were neutralized with the steroid antagonist RU-486. In contrast, LPS-treated neutrophil CM significantly increased neutrophil survival even after addition of polymyxin b. The survival effect of dexamethasone required the continuous presence of the agonist because addition of RU-486 caused prompt development of apoptosis in dexamethasone-treated cells. When naive and dexamethasone-treated cells were examined by mRNA differential display, a limited number of cDNA bands were consistently and reproducibly detected that were increased in intensity, indicating up-regulation by dexamethasone. Thus, corticosteroid regulation of neutrophil apoptosis is a specific effect that depends on continuous stimulation of synthesis of a (protein) survival factor.
This article has been cited by other articles:
|
|
 |
|
  J. A. Falk, O. A. Minai, and Z. Mosenifar Inhaled and Systemic Corticosteroids in Chronic Obstructive Pulmonary Disease Proceedings of the ATS, May 1, 2008; 5(4): 506 - 512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Saffar, M. P. Alphonse, L. Shan, K. T. HayGlass, F. E. R. Simons, and A. S. Gounni IgE Modulates Neutrophil Survival in Asthma: Role of Mitochondrial Pathway J. Immunol., February 15, 2007; 178(4): 2535 - 2541. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Madsen-Bouterse, G. J. M. Rosa, and J. L. Burton Glucocorticoid Modulation of Bcl-2 Family Members A1 and Bak during Delayed Spontaneous Apoptosis of Bovine Blood Neutrophils Endocrinology, August 1, 2006; 147(8): 3826 - 3834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Moisan and D. Girard Cell surface expression of intermediate filament proteins vimentin and lamin B1 in human neutrophil spontaneous apoptosis J. Leukoc. Biol., March 1, 2006; 79(3): 489 - 498. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Wesche-Soldato, J. L. Lomas-Neira, M. Perl, L. Jones, C.-S. Chung, and A. Ayala The role and regulation of apoptosis in sepsis Innate Immunity, December 1, 2005; 11(6): 375 - 382. [Abstract] [PDF]
|
|
|
|
|
|
M. G. Belvisi Regulation of Inflammatory Cell Function by Corticosteroids Proceedings of the ATS, November 1, 2004; 1(3): 207 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Lamote, E. Meyer, L. Duchateau, and C. Burvenich Influence of 17{beta}-Estradiol, Progesterone, and Dexamethasone on Diapedesis and Viability of Bovine Blood Polymorphonuclear Leukocytes J Dairy Sci, October 1, 2004; 87(10): 3340 - 3349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Matsuyama, M. Yamamoto, I. Higashimoto, K.-i. Oonakahara, M. Watanabe, K. Machida, T. Yoshimura, N. Eiraku, M. Kawabata, M. Osame, et al. TNF-related apoptosis-inducing ligand is involved in neutropenia of systemic lupus erythematosus Blood, July 1, 2004; 104(1): 184 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bouchard, C. Ratthe, and D. Girard Interleukin-15 delays human neutrophil apoptosis by intracellular events and not via extracellular factors: role of Mcl-1 and decreased activity of caspase-3 and caspase-8 J. Leukoc. Biol., May 1, 2004; 75(5): 893 - 900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 e. Moisan, e. Kouassi, and D. Girard Mechanisms involved in methylmercuric chloride (MeHgCl)-induced suppression of human neutrophil apoptosis Human and Experimental Toxicology, December 1, 2003; 22(12): 629 - 637. [Abstract] [PDF]
|
|
|
|
|
|
M. Choi, S. Rolle, M. Wellner, M. C. Cardoso, C. Scheidereit, F. C. Luft, and R. Kettritz Inhibition of NF-{kappa}B by a TAT-NEMO-binding domain peptide accelerates constitutive apoptosis and abrogates LPS-delayed neutrophil apoptosis Blood, September 15, 2003; 102(6): 2259 - 2267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tsuchihashi, K. Oishi, H. Yoshimine, S. Suzuki, A. Kumatori, T. Sunazuka, S. Omura, K. Matsushima, and T. Nagatake Fourteen-Member Macrolides Suppress Interleukin-8 Production but Do Not Promote Apoptosis of Activated Neutrophils Antimicrob. Agents Chemother., April 1, 2002; 46(4): 1101 - 1104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Stankova, S. Turcotte, J. Harris, and M. Rola-Pleszczynski Modulation of Leukotriene B4 Receptor-1 Expression by Dexamethasone: Potential Mechanism for Enhanced Neutrophil Survival J. Immunol., April 1, 2002; 168(7): 3570 - 3576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Strickland, K. Kisich, P. J. Hauk, A. Vottero, G. P. Chrousos, D. J. Klemm, and D. Y.M. Leung High Constitutive Glucocorticoid Receptor {beta} in Human Neutrophils Enables Them to Reduce Their Spontaneous Rate of Cell Death in Response to Corticosteroids J. Exp. Med., February 26, 2001; 193(5): 585 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Savoie, V. Lavastre, M. Pelletier, T. Hajto, K. Hostanska, and D. Girard Activation of human neutrophils by the plant lectin Viscum album agglutinin-I: modulation of de novo protein synthesis and evidence that caspases are involved in induction of apoptosis J. Leukoc. Biol., December 1, 2000; 68(6): 845 - 853. [Abstract] [Full Text]
|
|
|
|
|
|
A. C. Chin, W. D. Lee, K. A. Murrin, D. W. Morck, J. K. Merrill, P. Dick, and A. G. Buret Tilmicosin Induces Apoptosis in Bovine Peripheral Neutrophils in the Presence or in the Absence of Pasteurella haemolytica and Promotes Neutrophil Phagocytosis by Macrophages Antimicrob. Agents Chemother., September 1, 2000; 44(9): 2465 - 2470. [Abstract] [Full Text]
|
|
|
|
|
|
H. J. Chae, S. W. Chae, J. S. Kang, B. G. Bang, S. B. Cho, R. K. Park, H. S. So, Y. K. Kim, H. M. Kim, and H. R. Kim Dexamethasone Suppresses Tumor Necrosis Factor-{alpha}-Induced Apoptosis in Osteoblasts: Possible Role for Ceramide Endocrinology, August 1, 2000; 141(8): 2904 - 2913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Evans-Storms and J. A. Cidlowski Delineation of an Antiapoptotic Action of Glucocorticoids in Hepatoma Cells: The Role of Nuclear Factor-{kappa}B Endocrinology, May 1, 2000; 141(5): 1854 - 1862. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yoshiie, H.-Y. Kim, J. Mott, and Y. Rikihisa Intracellular Infection by the Human Granulocytic Ehrlichiosis Agent Inhibits Human Neutrophil Apoptosis Infect. Immun., March 1, 2000; 68(3): 1125 - 1133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. LEE, T. LINDO, N. JACKSON, L. MENG-CHOONG, P. REYNOLDS, A. HILL, M. HASWELL, S. JACKSON, and S. KILFEATHER Reversal of Human Neutrophil Survival by Leukotriene B4 Receptor Blockade and 5-Lipoxygenase and 5-Lipoxygenase Activating Protein Inhibitors Am. J. Respir. Crit. Care Med., December 1, 1999; 160(6): 2079 - 2085. [Abstract] [Full Text]
|
|
|
|
|
|
R. William, G. Watson, O. D. Rotstein, J. Parodo, R. Bitar, and J. C. Marshall The IL-1{beta}-Converting Enzyme (Caspase-1) Inhibits Apoptosis of Inflammatory Neutrophils Through Activation of IL-1{beta} J. Immunol., July 15, 1998; 161(2): 957 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Martin, I. Dransfield, C. Haslett, and A. G. Rossi Cyclic AMP Regulation of Neutrophil Apoptosis Occurs via a Novel Protein Kinase A-independent Signaling Pathway J. Biol. Chem., November 21, 2001; 276(48): 45041 - 45050. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
