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(Journal of Leukocyte Biology. 2002;72:147-153.)
© 2002 by Society for Leukocyte Biology

Sorting of neutrophil-specific granule protein human cathelicidin, hCAP-18, when constitutively expressed in myeloid cells

Elinor Bülow*, Niklas Bengtsson*, Jero Calafat{dagger}, Urban Gullberg* and Inge Olsson*

* Department of Hematology, Lund University, Sweden; and
{dagger} The Netherlands Cancer Institute, Amsterdam

Correspondence: Elinor Bülow, Dept. of Hematology, BMC, C14, S-221 84 Lund, Sweden. E-mail: elinor.bulow{at}hematologi.lu.se

Neutrophil granulocytes carry storage organelles, e.g., azurophil and specific granules. Poorly understood are the mechanisms for retrieval from constitutive secretion followed by sorting for storage. Therefore, we asked whether the specific granule protein human cathelicidin (hCAP-18) could be sorted for storage in other granules when the biosynthetic window is widened to allow this. We observed that hCAP-18 was targeted for storage in lysosome-related organelles when expressed constitutively in the rat basophilic leukemia and the mouse promyelocytic (MPRO) cell lines. In addition, premature release of the antibiotic C-terminal peptide LL-37 was observed. Retention of hCAP-18 was diminished by induction of differentiation of MPRO cells. In conclusion, a specific granule protein with native conformation may be sorted for storage in lysosome-related organelles of myeloid cells and converted prematurely to a supposedly biologically active form.

Key Words: secretory pathway • storage • LL-37 • lysosome-related organelle • azurophil granule




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J. Stie, A. V. Jesaitis, C. I. Lord, J. M. Gripentrog, R. M. Taylor, J. B. Burritt, and A. J. Jesaitis
Localization of hCAP-18 on the surface of chemoattractant-stimulated human granulocytes: analysis using two novel hCAP-18-specific monoclonal antibodies
J. Leukoc. Biol., July 1, 2007; 82(1): 161 - 172.
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